Capabilities of Saarum Sciences
Capability | Source |
Cell enrichment using FACS, MACS | Fresh tissues / blood |
Isolation and culture of primary cells | Fresh tissues / Blood |
Primary cell immortalization | Primary human cells |
Cell survival/ cytotoxicity assays/ Apoptosis | Fresh cells |
Delineating cellular pathways of interest | Fresh cells using FACS, Western blot analysis |
Target identification/ validation | IHC using FFPE slides Western blot analysis using flash frozen tissue |
Phenotypic assays for cancer metastasis | Primary and immortalized cells isolated from patients |
Assays for pro- and anti-inflammatory cytokines | PBMCs / other cell types isolated from healthy or diseased individuals |
Reporter assays for target genes | Primary and immortalized cells from patients |
Biochemical assays for specific molecules of interest | Serum, Plasma, Urine, and Saliva |
DNA / RNA extraction, PCR, qPCR | Blood, Fresh frozen tissues, FFPE, Saliva |